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American Society of Microbiology ABMM Sample Questions:

1. During a primary infection with Epstein-Barr virus (EBV), the virus establishes latency in B lymphocytes.
The transition from lytic to latent infection is tightly regulated by viral gene expression. Which of the following viral proteins is crucial for the establishment and maintenance of EBV latency and is a key target in EBV-associated lymphomas?

A) Early antigen (EA)
B) Epstein-Barr nuclear antigen 1 (EBNA1)
C) Glycoprotein gp350
D) Viral capsid antigen (VCA)

2. A microbiology laboratory is investigating a possible outbreak of Salmonella infection. Serological testing of patient isolates reveals they all possess the same O antigen but different H antigens. This suggests:

A) Contamination in the laboratory affecting multiple samples.
B) Multiple sources of infection with unrelated Salmonella strains.
C) A common ancestral strain that has undergone flagellar antigen variation.
D) A single point source of infection with a genetically stable strain.

3. The human virome, the collection of viruses that reside in and on the human body, is increasinglyrecognized for its role in health and disease. Which of the following statements regarding the human virome is MOST accurate?

A) The human virome is largely independent of the host's immune status.
B) Bacteriophages, viruses that infect bacteria, are a significant component of the human gut virome.
C) The human virome is primarily composed of pathogenic viruses that cause acute infections.
D) The composition of the human virome is relatively stable over time and is not influenced by environmental factors.

4. A clinical microbiology laboratory is performing Gram staining on a sputum sample. After adding the crystal violet stain, a delay in the washing step with decolorizer (alcohol-acetone) would MOST likely result in:

A) Increased background staining.
B) Gram-positive bacteria appearing pink.
C) Poor adherence of the stain to Gram-positive bacteria.
D) Gram-negative bacteria appearing purple.

5. A microbiology laboratory is performing Gram staining on a bacterial smear. If the safranin counterstain is omitted, Gram-negative bacteria will appear:

A) Purple
B) Blue
C) Brown
D) Colorless

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