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American Society of Microbiology ABMM Sample Questions:

1. A foodborne outbreak is suspected after several individuals who attended a potluck dinner develop nausea, vomiting, and abdominal cramps within 1-6 hours of eating. No fever or diarrhea is reported. The MOST likely bacterial toxin involved is:

A) Shiga toxin
B) Botulinum toxin
C) Clostridium perfringens enterotoxin
D) Staphylococcal enterotoxin

2. A microbiology laboratory is performing antimicrobial susceptibility testing using Etest strips. The MIC value is determined by:

A) The diameter of the zone of inhibition around the strip.
B) The presence or absence of growth at a single critical concentration.
C) The lowest concentration at which the elliptical zone of inhibition intersects the strip.
D) The highest concentration that allows visible bacterial growth within the zone of inhibition.

3. A research laboratory is investigating the mechanism of action of a novel antiviral compound that inhibits the replication of a specific RNA virus. They find that the compound binds directly to the viral RNA-dependent RNA polymerase, preventing its elongation activity. This mechanism is most similar to that of:

A) Oseltamivir
B) Interferon-beta
C) Acyclovir
D) Ribavirin

4. A patient with a history of travel to a developing country presents with chronic diarrhea, abdominal pain, and weight loss. Stool examination reveals the presence of cysts containing four nuclei and chromatoidal bars with rounded ends. The MOST likely causative organism is:

A) Entamoeba histolytica
B) Cryptosporidium parvum
C) Giardia lamblia
D) Cyclospora cayetanensis

5. In a clinical microbiology laboratory, quality control testing of Mueller-Hinton agar used for disk diffusion susceptibility testing involves checking the zone sizes for E. coli ATCC 25922, S. aureus ATCC 25923, and
P. aeruginosa ATCC 27853. What is the primary purpose of including P. aeruginosa ATCC 27853 in this QC?

A) To confirm the correct pH of the agar medium
B) To ensure appropriate concentration of calcium and magnesium ions affecting aminoglycoside activity
C) To verify the performance of cell wall active agents like penicillin
D) To assess the performance of agents primarily active against Gram-positive organisms

Solutions: