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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Hematology | 20-25% | - Routine and automated hematology procedures - White blood cell morphology and diseases - Hemostasis and coagulation testing - Red blood cell physiology and disorders |
| Topic 2: Clinical Chemistry | 20-25% | - Carbohydrates, lipids, proteins, and enzymes - Toxicology and liver/kidney function tests - Electrolytes, acid-base balance, and blood gases - Endocrinology and therapeutic drug monitoring |
| Topic 3: Blood Banking (Immunohematology) | 15-20% | - Blood group systems and antigens - Antibody detection and identification - Transfusion reactions and adverse events - Compatibility testing - Blood components and storage |
| Topic 4: Laboratory Operations | 5-10% | - Pre-analytical, analytical, and post-analytical processes - Quality control and quality assurance - Laboratory safety and regulatory compliance - Laboratory mathematics and instrumentation |
| Topic 5: Immunology and Serology | 5-10% | - Autoimmune and immunodeficiency disorders - Immune system principles and components - Immunologic testing methodologies - Infectious disease serology |
| Topic 6: Urinalysis and Other Body Fluids | 5-10% | - Cerebrospinal, serous, and synovial fluids - Physical, chemical, and microscopic analysis - Urine formation and composition - Semen and amniotic fluid testing |
| Topic 7: Microbiology | 15-20% | - Bacteriology and antimicrobial susceptibility - Specimen collection and culture techniques - Identification of pathogenic microorganisms - Mycology, parasitology, and virology |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
1. The clue to the identification of the colony seen in the upper frame is the gram stain in the lower frame, in which are seen short, rounded, gram positive bacilli, many of which possess distinct spores. As the colonies grew aerobically, the presence of spores indicates Bacillus species. The colonies are spreading, smooth, yellow-white and non-hemolytic. The lack of hemolysis and the small size of the bacterial cells suggests a species other than Bacillus cereus, the species causing most human infections. The isolate was identified as Bacillus circulans, which is consistent with the gram stain morphology. Clostridium septicum also produces spores; however, this species is an anaerobe and would grow poorly if at all and not produce spores aerobically. Listeria monocytogenes and Lactobacillus species are gram positive bacilli; however, neither of these produce spores.
The colonies seen in the upper frame, grown after 48 hours incubation aerobically at 35°C, and the accompanying gram stain in the lower frame are uncommonly associated with human disease, but have been associated with septicemia, bronchopneumonia, osteomyelitis and other infections, particularly in intravenous drug users. The most likely identification is:
A) Lactobacillus species
B) Clostridium septicum
C) Bacillus circulans
D) Listeria monocytogenes
2. "Universal donor", (a misnomer) is usually applied to group O, Rh negative blood. Although it may be necessary to use group O, Rh negative blood in an extreme emergency, it is preferable to use type specific blood for emergencies.
In an extreme emergency , if the ABO and Rh type are unknown which of the following should be given to the patient?
A) Any blood type is OK
B) Group O, Rh positive blood
C) Group O, Rh negative blood
D) Group AB, Rh negative blood
3. Which one of the following conditions is NOT associated with hereditary spherocytosis?
A) Intravascular hemolysis
B) Extravascular hemolysis
C) An MCHC greater than 37%
D) Increased osmotic fragility
4. The division of Department of Health and Human Services (DHHS) responsible for protecting public health by ensuring the safety and efficacy of foods, drugs, biological products, medical devices, and cosmetics is:
A) Clinical and Laboratory Standards Institute (CLSI)
B) the Joint Commission
C) Food and Drug Administration (FDA)
D) Health Insurance Portability and Accountability Act (HIPPA)
5. Enterococcus spp. are bile esculin positive and grow in 6.5% NaCl. If they grow on the 6 µg/mL vancomycin screen agar as this isolate does, it is vancomycin resistant. If there is no growth on the 6 µg/mL vancomycin screen agar, the isolate is susceptible to vancomycin. Streptococcus Group D, not Enterococcus will not grow in the 6.5% NaCl and should not be tested for vancomycin resistance. Staphylococcus epidermidis is catalase positive.
The images below represent a gram-positive, catalase-negative, non-motile coccus inoculated to bile esculin agar, 6.5% NaCl broth and to 6 µg/mL of vancomycin in Mueller Hinton agar. It should be reported as:
A) Staphylococcus epidermidis, Resistant to vancomycin
B) Enterococcus spp., Susceptible to vancomycin
C) Streptococcus Group D, not Enterococcus
D) Enterococcus spp., Resistant to vancomycin
Solutions:
| Question # 1 Answer: C | Question # 2 Answer: C | Question # 3 Answer: A | Question # 4 Answer: C | Question # 5 Answer: D |
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